Zoinks! Two years after a clash of egos forced Mystery Inc. to close its doors, Scooby-Doo and his clever crime-solving cohorts Fred (FREDDIE PRINZE JR.), Daphne (SARAH MICHELLE GELLAR), Shaggy (MATTHEW LILLARD) and Velma (LINDA CARDELLINI) are individually summoned to Spooky Island to investigate a series of paranormal incidents at the ultra-hip Spring Break hot spot.
Concerned that his way cool theme part might truly be haunted, Spooky Island owner Emile Mondavarious (ROWAN ATKINSON) tries to reunite those notoriously meddling detectives to solve the mystery before his supernatural secret scares away the college crowds.
Scooby and the gang will have to overcome their personal differences and forget everything they think they know about fake ghouls and phony creatures to crack the case, save themselves and possibly the world. RUH-ROH!
| Freddie Prinze, Jr. | Fred |
| Sarah Michelle Gellar | Daphne |
| Matthew Lillard | Shaggy |
| Linda Cardellini | Velma |
| Rowan Atkinson | Mondavarious |
| Isla Fisher | Mary Jane |
| Miguel A. Nuñez, Jr. | Voodoo Maestro |
| Steven Grives | N' Goo Tuana |
| Charles Stan Frazier | |
| Craig Bullock | |
| Matthew Murphy Karges | |
| Mark McGrath | |
| Rodney Sheppard | |
| Sam Greco | Zarkos |
| Charles Cousins | Velma's Friend |
| Kristian Schmid | Brad |
| Nicholas Hope | Old Man Smithers |
| Neil Fanning | Scooby Voice |
| Scott Innes | Scrappy Voice |
| J. P. Manoux | Scrappy Rex Voice |
| Chris Cruickshanks | Tiny Henchman |
| Alex Ruiz | Reporter #1 |
| Sheryl Benko | Reporter #2 |
| Rio Nugara | Island Emissary |
| David Vallon | Bartender Dead Mike's |
| Troy MacKinder | Guy in the Vat |
| Michala Banas | Carol |
| Holly Brisley | Training Video Woman |
| Robert Diaz | Training Video Guy #1 |
| Remi Broadway | Training Video Guy #2 |
| Martin Broome | Melvin Doo |
| Simone Dumbleton | Co-Ed |
| Jonathan Coffey | Fitzgibbon |
| Michael Caffrey | Coast Guard #2 |
| Kyas Sherriff | Airport Attendant |
| Celeste Gosnell | |
| Bradley Gosnell | |
| Cayley Gosnell | |
| Audrey Gosnell | |
| Kurt Duval | Spooky Hotel Bartender |
| Janis McGavin | Co-Ed Hottie |
| Emily Gosnell | |
| Marea Lambert Barker | |
| Kym Jackson | |
| Danielle Starkey | |
| Andrew Bryniarski | Cavern Henchman |
| Craig Behenna | Henchman #1 |
| Keith S. Bullock | Henchman #2 |
| Jess Harnell | |
| Frank Welker | |
| Stephen Colyer | |
| Ashley Evans | |
| Mark Hodge | |
| Stephen Holford | |
| Matt Lee | |
| Michael Montgomery | |
| Deon Nuku | |
| Robert Ricks | |
| Rodney Syaranamual | |
| Ashley Wallen | |
| Andrew Waters | |
| Adam Williams | |
| Pamela Anderson | Herself |
| Robert Engelman | Executive Producer |
| Andrew Mason | Executive Producer |
| Kelley Smith-Wait | Executive Producer |
| William Hanna | Executive Producer |
| Joseph Barbera | Executive Producer |
| David Eggby | Director of Photography |
| Kent Beyda | Film Editor |
| David Newman | Composer |
| Paul Brincat | production sound mixer |
| Mike Wilhoit | Supervising Sound Editor |
| Scott Sanders | Sound Designer |
| John Reitz | Sound Re-Recording Mixer |
| Gregg Rudloff | Sound Re-Recording Mixer |
| David Campbell | Sound Re-Recording Mixer |
| Bill Boes | Production Designer |
| Leesa Evans | Costume Designer |
| Peter Crosman | Visual Effects |
| Bill Booth | Art Director |
| Donna Brown | Art Director |
| Gabrielle Gliniak | Art Director |
| Christian Wintter | Art Director |
| Robyn Elliot | Costume Designer |
| Lesley Vanderwalt | Make-Up Artist |
| Paul Pattison | Make-Up Artist |
| Lynn Wheeler | Make-Up Artist |
| Zeljka Stanin | Make-Up Artist |
| Annette Hardy | Make-Up Artist |
| Brian Cox | Visual Effects |
| Gabriella Nagy | Visual Effects |
| Max Fletcher | Visual Effects |
| Walter Van Veenendaal | Visual Effects |
| Aaron Beaucaire | Visual Effects |
| Paul Gorrie | Visual Effects |
| Angelo Sahin | Visual Effects |
| Kevin Turner | Visual Effects |
| Steve Szekeres | Visual Effects |
| Kim Hilder | Visual Effects |
