Jungle Cruise is een spannende rit over de Amazone met de komische kapitein Frank Wolff en de onverschrokken onderzoeker Dr. Lily Houghton. Lily reist van Londen naar het Amazone-oerwoud en doet een beroep op de diensten van Frank om haar op La Quila, zijn gammele maar charmante boot, te vervoeren. Lily is vastberaden om een oeroude boom met helende krachten te vinden om de toekomst van de geneeskunde te veranderen. Samen op deze spannende zoektocht stuit het verrassende duo op eindeloze gevaren en bovennatuurlijke krachten, die zich verborgen houden in de misleidende schoonheid van het weelderige regenwoud. Maar terwijl de geheimen van de verloren boom zich openbaren, worden de risicos voor Lily en Frank nog groter want hun lot, en dat van de mensheid, staat op het spel.
| Dwayne Johnson | Frank Wolff |
| Emily Blunt | Lily Houghton |
| Édgar Ramírez | Aguirre |
| Jack Whitehall | MacGregor Houghton |
| Jesse Plemons | Prince Joachim |
| Paul Giamatti | Nilo |
| Veronica Falcón | Trader Sam |
| Dani Rovira | Sancho |
| Quim Gutiérrez | Melchor |
| Dan Dargan Carter | Gonzalo |
| Andy Nyman | Sir James Hobbs-Coddington |
| Raphael Alejandro | Zaqueu |
| Simone Lockhart | Anna |
| Pedro Lopez | Chief |
| Sulem Calderon | Chief's daughter |
| Sebastian Blunt | Society Guard |
| Mark Ashworth | Society Member |
| Allan Poppleton | Society Worker |
| Caroline Paige | Kid Tourist |
| James Quattrochi | Italian Tourist |
| Stephen Dunlevy | Middlepart |
| Philipp Maximilian | Axel |
| Romualdo Castillo | Animal Vendor |
| Pedro Haro | Bird Vendor |
| Christina Souza | Barmaid |
| Michael H. Cole | Bus Conductor |
| Hector Banos | |
| Peter Zimmerman | |
| Travis Gomez | |
| Ismael Herrera | |
| David Lengel | |
| Justin Randell Brooke | |
| Victoria Blade | |
| Brooke Jaye Taylor | |
| Vince Pisani | |
| Piper Collins | |
| Keith Arthur Bolden | |
| Chip Steele | |
| Ben Jenkin | Proxima |
| David Paris | |
| Kevin LaRosa | |
| Remington Steele | |
| Kevin Edward Morgan | |
| Reid Harper | |
| Trent Brya | |
| Max Calder | |
| Greg Tresan | Horse Conquistador |
| Scott Sheldon | Executive Producer |
| Doug Merrifield | Executive Producer |
| Flavio Labiano | Director of Photography |
| Joel Negron | Film Editor |
| James Newton Howard | Composer |
| Ethan Van der Ryn | Supervising Sound Editor |
| Erik Aadahl | Supervising Sound Editor |
| Ron Bartlett | Sound Re-Recording Mixer |
| Doug Hemphill | Sound Re-Recording Mixer |
| Michael B. Koff | production sound mixer |
| John Marquis | Sound Designer |
| Brandon Jones | Sound Designer |
| Jean-Vincent Puzos | Production Designer |
| Paco Delgado | Costume Designer |
| Jim Berney | Visual Effects |
| Jake Morrison | Visual Effects |
| David Lazan | Art Director |
| Greg Hooper | Art Director |
| Drew Monahan | Art Director |
| Thomas P. Wilkins | Art Director |
| Stacy M. Horn | Costume Designer |
| Joel Harlow | Make-Up Artist |
| Merc Arceneaux | Make-Up Artist |
| Evelyne Noraz | Make-Up Artist |
| Kimberly Felix-Burke | Make-Up Artist |
| Ozzy Alvarez | Make-Up Artist |
| Mark Ross | Make-Up Artist |
| André Freitas | Make-Up Artist |
| Addison Foreman | Make-Up Artist |
| Devin Morales | Make-Up Artist |
| Rachel Bongard | Make-Up Artist |
| Derek Garcia | Make-Up Artist |
| Jordan Venetis | Make-Up Artist |
| Matt Sprunger | Make-Up Artist |
| J. D. Schwalm | Visual Effects |
| Luke Millar | Visual Effects |
| Chad Wiebe | Visual Effects |
| Alex Prichard | Visual Effects |
| John Knoll | Visual Effects |
| Ara Khanikian | Visual Effects |
| Chris Downs | Visual Effects |
| Tim McGovern | Visual Effects |
| Malte Sarnes | Visual Effects |
| Viktor Muller | Visual Effects |
| Gaia Bussolati | Visual Effects |
