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| Colin Clive | Henry Frankenstein |
| Robert De Niro | The Creature |
| Mae Clarke | Elizabeth |
| Kenneth Branagh | Victor Frankenstein |
| Tom Hulce | Henry Clerval |
| John Boles | Victor Moritz |
| Helena Bonham Carter | Elizabeth |
| Boris Karloff | The Monster (as ?) |
| Aidan Quinn | Captain Robert Walton |
| Edward Van Sloan | Doctor Waldman |
| Ian Holm | Baron Frankenstein |
| Frederick Kerr | Baron Frankenstein |
| Richard Briers | Grandfather |
| Dwight Frye | Fritz |
| John Cleese | Professor Waldman |
| Lionel Belmore | The Burgomaster |
| Robert Hardy | Professor Krempe |
| Marilyn Harris | Little Maria |
| Bernhard Kaun | Composer |
| Cherie Lunghi | Caroline Beaufort Frankenstein |
| Arthur Edeson | Cinematographer |
| Celia Imrie | Mrs. Moritz |
| Paul Ivano | Cinematographer |
| Trevyn McDowell | Justine |
| Clarence Kolster | Editor |
| Gerard Horan | Claude |
| Jimmy Yuill | |
| Mark Hadfield | Felix |
| Hugh Bonneville | |
| Joanna Roth | Marie |
| Shaun Prendergast | |
| Gary Oldman | |
| Patrick Doyle | Composer |
| Sasha Hanau | Maggie |
| David Kennedy | |
| Winona Ryder | |
| Roger Pratt | Cinematographer |
| Joseph England | Thomas |
| Siobhan Redmond | |
| Anthony Hopkins | |
| Andrew Marcus | Editor |
| Alfred Bell | Landlord |
| Angus Wright | |
| Jim Carter | |
| Richard Clifford | Minister |
| Aaron Eckhart | Adam |
| George Asprey | Policeman |
| Yvonne Strahovski | Terra |
| Miranda Otto | Leonore |
| Ryan J-W Smith | William |
| Bill Nighy | Naberius |
| Charles Wyn-Davies | Young William |
| Jai Courtney | Gideon |
| Rory Jennings | Young Victor |
| Socratis Otto | Zuriel |
| Christina Cuttall | Young Justine |
| Aden Young | Victor Frankenstein |
| Hannah Taylor Gordon | Young Elizabeth |
| Caitlin Stasey | Keziah |
| Susan Field | Frau Brach |
| Mahesh Jadu | Ophir |
| Chris Barnes | Ships Crew |
| Steve Mouzakis | Helek |
| Nicholas Bell | Carl Avery |
| Deniz Akdeniz | Barachel |
| Chris Pang | Levi (as Christopher Pang) |
| Kevin Grevioux | Dekar |
| Bruce Spence | Molokai |
| Virginie Le Brun | Elizabeth Frankenstein |
| Penny Higgs | Sargon |
| Goran D. Kleut | Rekem (as Goran Kleut) |
| Yasca Sinigaglia | Igal |
| Nicole Downs | Procula |
| Lonnie James | Rough Woman |
| John Reynolds | Jannes |
| Jenny Galloway | Vendor's Wife |
| Rick Tonna | Bar Bouncer |
| Peter Jonfield | Rough Man |
| Craig Brookshaw | Alley Demon #1 |
| Edward Jewesbury | City Official |
| Bryce Hardy | Alley Demon #2 |
| Marky Lee Campbell | Police Officer |
| Warwick Sadler | Forest Demon #1 (as Warrick Sadler) |
| Robbie Clissold | Forest Demon #2 |
| Jack Fieguth | Forest Demon #3 |
| Michael Peace | Subway Lover #1 |
| Sherrydayne Taela | Subway Lover #2 |
| Chris Anderson | Train Station Cleaner |
| Jim Petropoulos | Wessex Orderly |
| Tim Harvey | Production Designer |
| Martin Childs | Art Director |
| Desmond Crowe | Art Director |
| John Fenner | Art Director |
| James Acheson | Custome Designer |

