It is 1942, America has entered World War II, and sickly but determined Steve Rogers is frustrated at being rejected yet again for military service. Everything changes when Dr. Erksine recruits him for the secret Project Rebirth. Proving his extraordinary courage, wits and conscience, Rogers undergoes the experiment and his weak body is suddenly enhanced into the maximum human potential. When Dr. Erksine is then immediately assassinated by an agent of Nazi Germany's head of its secret HYDRA research department, Johann Schmidt aka the Red Skull, Rogers is left as a unique man who is initially misused as a propaganda mascot. However, when his comrades need him, Rogers goes on a successful adventure that truly makes him Captain America and his war against Schmidt begins.
| Chris Evans | Captain America |
| Hayley Atwell | Peggy Carter |
| Sebastian Stan | James Buchanan 'Bucky' Barnes |
| Tommy Lee Jones | Colonel Chester Phillips |
| Hugo Weaving | Johann Schmidt |
| Dominic Cooper | Howard Stark |
| Richard Armitage | Heinz Kruger |
| Stanley Tucci | Dr. Abraham Erskine |
| Samuel L. Jackson | Nick Fury |
| Toby Jones | Dr. Arnim Zola |
| Neal McDonough | Timothy 'Dum Dum' Dugan |
| Derek Luke | Gabe Jones |
| Kenneth Choi | Jim Morita |
| JJ Feild | James Montgomery Falsworth |
| Bruno Ricci | Jacques Dernier |
| Lex Shrapnel | Gilmore Hodge |
| Michael Brandon | Senator Brandt |
| Martin Sherman | Brandt's Aide (as Martin T. Sherman) |
| Natalie Dormer | Pvt. Lorraine |
| Oscar Pearce | Search Team Leader |
| William Hope | SHIELD Lieutenant |
| Nicholas Pinnock | SHIELD Tech |
| Marek Oravec | Jan |
| David Bradley | Tower Keeper |
| Leander Deeny | Steve Rogers Double / Barman |
| Sam Hoare | Nervous Recruit |
| Simon Kunz | 4F Doctor |
| Kieran O'Connor | Loud Jerk |
| Jenna-Louise Coleman | Connie |
| Sophie Colquhoun | Bonnie |
| Doug Cockle | Young Doctor |
| Ben Batt | Enlistment Office MP |
| Mollie Fitzgerald | Stark Girl |
| Damon Driver | Sergeant Duffy |
| David McKail | Johann Schmidt's Artist |
| Amanda Walker | Antique Store Owner |
| Richard Freeman | SSR Doctor |
| Katherine Press | Project Rebirth Nurse |
| Sergio Corvino | Kruger's Aide |
| Marcello Marascalchi | Undercover Bum (as Marcello Walton) |
| Marcello Walton | Undercover Bum |
| Vincent Montuel | Undercover Bum |
| Fabrizio Santino | Kruger's Driver |
| Maxwell Newman | Boy at Dock |
| Anatole Taubman | Roeder |
| Jan Pohl | Hutter |
| Erich Redman | Schneider |
| Rosanna Hoult | The Star Spangled Singer |
| Naomi Slights | The Star Spangled Singer |
| Kirsty Mather | The Star Spangled Singer |
| Megan Sanderson | Kid in USO Audience |
| Darren Simpson | Kid in USO Audience |
| Fernanda Toker | Newsstand Mom |
| Laura Haddock | Autograph Seeker |
| James Payton | 'Adolph Hitler' |
| Ronan Raftery | Army Heckler |
| Nick Hendrix | Army Heckler |
| Luke Allen-Gale | Army Heckler |
| Jack Gordon | Army Heckler |
| Ben Uttley | HYDRA Guard |
| Kevin Millington | Stark's Engineer |
| Patrick Monckeberg | Manager Velt |
| Peter Stark | HYDRA Lieutenant |
| Amanda Righetti | SHIELD Agent |
| Chris Jorie | Reporter (as Chris Jorie) |
| Jennifer Abbotts | USO Dancer |
| Michael Swan | Dodgers Announcer |
| Michelle Antrobus | USO Dancer |
| Lucy Dean | USO Dancer |
| Erin Dusek | USO Dancer |
| Nicole Evans | USO Dancer |
| Lizzi Franklin | USO Dancer |
| Emma Harris | USO Dancer |
| Rachael Isherwood | USO Dancer |
| Danielle Kelly | USO Dancer |
| Nicole May | USO Dancer |
| Stevie-Jean McGuire | USO Dancer |
| Anna McNicholas | USO Dancer |
| Rosie Pethullis | USO Dancer |
| Jessica Powell | USO Dancer |
| Rachel Quartley | USO Dancer |
| Sarah Riches | USO Dancer |
| Holly Rostron | USO Dancer |
| Caroline Royce | USO Dancer |
| Stephanie Jayne Thompson | USO Dancer |
| Lucie Waugh | USO Dancer |
| Billy Lozowski | USO Drummer |
| Alan Silvestri | Composer |
| Gregory Paulett | USO Drummer |
| Shelly Johnson | Cinematographer |
| Adam Birch | USO Drummer |
| Robert Dalva | Editor |
| Adam Kent | USO Drummer |
| Jeffrey Ford | Editor |
| Wiliam Morris | USO Drummer (as William Morris) |
| Sarah Finn | Casting Director |
| David Sdall | USO Drummer |
| Randi Hiller | Casting Director |
| Paul Simmons | USO Drummer |
| Priscilla John | Casting Director |
